Isolation and Characterization of Endosymbiotic Bacteria Producing Biopigments from Dye-Yielding Plants in North Central Timor Regency

Abstract

Abstract

The use of biopigments has emerged as an alternative solution to minimize the risks associated with synthetic dyes. Biopigments, also known as natural dyes, can be extracted not only from plants but also from bacteria. Biopigments are biodegradable, making them more environmentally friendly and safe for health. This research is important to conduct because it supports one of the Sustainable Development Goals (SDGs), namely good health and well-being. The use of natural dyes provides a safer and more environmentally friendly solution for health. The research stages included isolation, molecular characterization of 16S rRNA, biochemical activity tests, and extraction and characterization of the types of pigments produced. The research results from 15 pure isolates selected four isolates with the most potential. Based on the results of staining gram and biochemical tests and molecular identification, the endosymbiotic bacterial isolate code HTB1 has a similarity to Pseudomonas putida strain OS-18, isolate code HTB2 has a similarity to Pantoea sp, and isolate code HTD2 has a similarity to Bacillus subtilis strain G-13. Yellow pigment was extracted by the solvent extraction method and characterized by using UV–Vis spectroscopy and Fourier-transform infrared spectroscopy. The Maximum absorbance was obtained at 452 nm, justify the presence of carotenoid pigment, and FTIR results reveal the same.